Inonotus hispidus (I. hispidus) is a medicinal macrofungus that plays a key role in anti-tumor and antioxidant functions. To further understand and enhance the value of I. hispidus, we performed whole-genome sequencing and an analysis of its strain for the first time. I. hispidus was sequenced using the Illumina NovaSeq high-throughput sequencing platform. The genome length was 35,688,031 bp and 30 contigs, with an average length of 1,189,601.03 bp. Moreover, database alignment annotated 402 CAZyme genes and 93 functional genes involved in regulating secondary metabolites in the I. hispidus genome to find the greatest number of genes involved in terpenes in that genome, thus providing a theoretical basis for its medicinal value. Finally, the phylogenetic analysis and comparative genomic analysis of single-copy orthologous protein genes from other fungi in the same family were conducted; it was found that I. hispidus and Sanghuangporus baumii have high homology. Our results can be used to screen candidate genes for the nutritional utilization of I. hispidus and the development of high-yielding and high-quality I. hispidus via genetic means.
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机译：Inonotus hispidus （I. hispidus） 是一种药用大型真菌，在抗肿瘤和抗氧化功能中起关键作用。为了进一步了解和提高 I. hispidus 的价值，我们首次对其菌株进行了全基因组测序和分析。使用 Illumina NovaSeq 高通量测序平台对 I. hispidus 进行测序。基因组长度为 35,688,031 bp，有 30 个重叠群，平均长度为 1,189,601.03 bp。此外，数据库对齐注释了 402 个 CAZyme 基因和 93 个参与调控 Hispidus 基因组次生代谢产物的功能基因，以找到该基因组中涉及萜烯的基因数量最多的基因，从而为其药用价值提供理论依据。最后，对来自同一家系其他真菌的单拷贝直系同源蛋白基因进行系统发育分析和比较基因组分析;结果发现 I. hispidus 和 Sanghuangporus baumii 具有高度的同源性。我们的结果可用于筛选 I. hispidus 营养利用的候选基因，并通过遗传手段开发高产和高质量的 I. hispidus。
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