The aim of this study was to analyze the match-to-match variation in high-intensity demands from one Portuguese professional football team according to playing positions. Twenty-three male outfield professional football players were observed during eighteen matches of the Portuguese Second League. Time–motion data were collected using Global Positioning System (GPS) technology. Match running performance was analyzed based on the following three playing positions: defenders (DF), midfielders (MF), and forwards (FW). Repeated measures ANOVA was utilized to compare match running performance within each position role, and seasonal running variation. Practical differences were assessed using the smallest worthwhile change (SWC), coefficient of variation (CV), and twice the coefficient of variation (2CV). Significant differences were found among playing positions in total distance covered (F = 15.45, p < 0.001, η2 = 0.33), average speed (F = 12.79, p < 0.001, η2 = 0.29), high-speed running (F = 16.93, p < 0.001, η2 = 0.36), sprinting (F = 13.49, p < 0.001, η2 = 0.31), accelerations (F = 4.69, p = 0.001, η2 = 0.132), and decelerations (F = 12.21, p < 0.001, η2 = 0.284). The match-to-match running performance encompassed TD (6.59%), AvS (8.67%), HSRr (37.83%), SPR (34.82%), ACC (26.92%), and DEC (27.85%). CV values for total distance covered ranged from 4.87–6.82%, with forwards and midfielders exhibiting the greatest and smallest variation, respectively. Midfielders demonstrated the highest match-to-match variation for all other analyzed variables (8.12–69.17%). All playing positions showed significant variation in high-demanding variables (26.94–37.83%). This study presents the initial analysis of match-to-match variation in high-intensity demands within a Portuguese professional football team. Thus, the position’s specificity and context can provide a helpful strategy for evaluating match-to-match running performance, and for recommending individualized training exercises based on the peak and high-intensity demands for each player’s role within the game.
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