Examination of the Transcriptional Response to LaMIR166a Overexpression in Larix kaempferi (Lamb.) Carr

摘要

The study of somatic embryogenesis can provide insights into early plant development. To elucidate the molecular mechanisms associated with miR166 in Larix kaempferi (Lamb.) Carr, the transcriptional profiles of wild-type (WT) and LaMIR166a-overexpressing embryonic cells were subjected to RNA sequencing. In total, 2467 differentially expressed genes were obtained. The cleaved degree of LaHDZ31–34 was higher in transgenic lines than in WT. The genes related to LaHDZ31–34 were screened by transcriptome analysis, and a yeast one-hybrid and dual-luciferase report assay revealed that LaHDZ31–34 could bind to the promoters of LaPAP, LaPP1, LaZFP5, and LaPHO1. This study provides insights into the regulatory mechanisms of miR166.