Deletion of the mitochondria-shaping protein Opa1 during early thymocyte maturation impacts mature memory T cell metabolism

摘要

A Quantification of mitochondrial mass measured with MitoTracker Green in WT thymocytes analyzed by flow cytometry. Data are mean ± SEM (N = 3). B Quantification of mitochondrial membrane potential measured with TMRM in WT thymocytes analyzed by flow cytometry. Data are mean ± SEM (N = 3). C Mitochondrial membrane potential/mitochondrial mass ratio expressed as TMRM/Mitotracker green ratio in WT;Lck-Cre+ thymocytes analyzed by flow cytometry. Data are mean ± SEM (N = 3). D Oxygen consumption rate (OCR) of WT thymocytes at baseline and after exposure to vehicle/etomoxir, oligomycin (Oligo), FCCP, and rotenone/antimycin (Rot/Ant). Data are representative of three independent experiments (N = 4/group). E Extracellular acidification rate (ECAR) of WT thymocytes at baseline and after exposure to vehicle/etomoxir, oligomycin (Oligo), FCCP, and rotenone/antimycin (Rot/Ant). Data are representative of three independent experiments (N = 4/group). F SRC of WT thymocytes treated as in (D). Data are mean ± SEM (N = 4). G OCR/ECAR ratio of WT thymocytes treated as in (D). Data are mean ± SEM (N = 4). H WT DN3 and DN4 thymocytes were sorted and OCR was measured at baseline and after exposure to oligomycin (Oligo), FCCP, and rotenone/antimycin (Rot/Ant). Data are mean ± SEM (N = 3). I WT DN3 and DN4 thymocytes were sorted and ECAR was measured at baseline and after exposure to oligomycin (Oligo), FCCP, and rotenone/antimycin (Rot/Ant). Data are mean ± SEM (N = 3). J SRC of WT DN3 and DN4 thymocytes treated as in (E). Data are mean ± SEM (N = 3). K OCR/ECAR ratio of WT DN3 and DN4 thymocytes treated as in (E). Data are mean ± SEM (N = 3).