A simple solid‐phase extraction method for the analysis of red cell phospholipids by liquid chromatography‐ tandem mass spectrometry

摘要

There has been increasing interest in the analysis of phospholipids in red blood cells as potential long‐term biomarkers of different disease states. Here, we describe a simple method for the analysis of two phospholipids: 1‐Palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphoethanol (PE 16:0/18:1) and 1‐Palmitoyl‐2‐linoleoyl‐sn‐glycero‐3‐phosphoethanol (PE 16:/0/18:2) in erythrocytes by liquid chromatography‐tandem mass spectrometry (LC‐MS/MS).