Downregulated miR-18b-5p triggers apoptosis by inhibition of calcium signaling and neuronal cell differentiation in transgenic SOD1 (G93A) mice and SOD1 (G17S and G86S) ALS patients

摘要

SOD1 mutation (G93A) is related to altered gene expression and induces apoptosis. a The heat map of RNA-seq from subcellular fractionized wt and mtNSC-34 stable cells showed that relative expressions of Hif1α, Mef2c, Mctp1 and Rarb are altered by SOD1(G93A). The relative expression genes was displayed as colors: higher (red) or lower (green). The heat map represents the average of three samples. b RT-PCR analysis explained that Mctp1 and Rarb introns are upregulated in nucleus of mtNSC-34 cells and Mctp1 and Rarb exons are highly downregulated in cytoplasm of mtNSC-34 cells. c RT-qPCR analysis showed that Mctp1 and Rarb transcripts are decreased in mtNSC-34 cells. d miR-206 expression was induced in mtNSC-34 cells. e and f SOD1 aggregation (arrow) prevents neuronal differentiation (MAP2) and increases intracellular Ca2+ levels (WT (0.072) versus MT (0.108) in fluorescence intensities from baseline 490/525 ratio) in mtNSC-34 cells. Scale bar, 40 μm. Significantly different at *, p < 0.05; **, p < 0.005. The experiments were replicated 3 times