Carbon nanotube filler enhances incinerated thermoplastics-induced cytotoxicity and metabolic disruption in vitro

摘要

Microscopic assessment of Beas-2B cells treated with incinerated thermoplastics. Beas-2B treated with 0.6 μg/cm2 or 1.2 μg/cm2 incinerated for 48 h were visualized via enhanced darkfield microscopy (EDM) or transmission electron microscopy (TEM), respectively. Particles in suspension were also visualized by TEM for comparison against structures identified in Beas-2B cells. Incinerated thermoplastics visualized by EDM are identified by a bright spectral signature (Magnification: 60X, scale bar = 10 μm). Cells treated with incinerated thermoplastics were visualized under low magnification (Scale Bar = 4 μm) and high magnification (Yellow Box denotes region of high magnification, Scale Bar = 1 μm). Particle dispersed in water were prepared and visualized for TEM (Scale Bar = 1 μm). Endocytosed particles are identified by red arrows in EDM and high magnification TEM images