Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene

摘要

Schematic representation of truncations in the α-MAT secretory signal. a α-MAT consisting of the pre-region (19 amino acids), followed by a pro-region (66 amino acids) with three N-linked glycosylation sites (red box). Truncations, executed in the present study, in the pro-region of the α-MAT are shown. The position of the mutations is indicated by amino acid numbers b Schematic representation of the wild type and the truncated α-MAT (Δ57–70, shown as an example here) fused with the WT-GCSF. c Schematic representation of the wild type and the truncated α-MAT (Δ57–70, shown as an example here) fused with the CO-GCSF