Changes in relative histone abundance and heterochromatin in αA-crystallin and αB-crystallin knock-in mutant mouse lenses

摘要

MALDI-TOF MS analysis of histones isolated from adult mouse lenses. WT, wild type. The histone peaks were identified based on their mass to charge (m/z) ratios. The m/z peaks on the left side of the x-axis represent doubly-charged ions for histones H4 [H4/2]2+, H2 [H2/2]2+, and H3 [H3/2]2+, respectively. MALDI spectra usually deliver single charged ions, but can also produce doubly-charged ions, especially for proteins