Immune activation of murine RAW264.7 macrophages by sonicated and alkalized paramylon from Euglena gracilis

摘要

Paramylon triggers NO release in RAW264.7 macrophages. a After stimulation of macrophages with paramylon (10, 50, 100, and 200 μg/mL), in the presence or absence of the lipopolysaccharide-inhibitor polymyxin B (PMB), the NO production in the culture supernatant was measured with Griess reagent. b Cell viability, was measured with a cell counting kit. c Paramylon dose responses. NO production in the culture supernatant was measured using Griess reagent as in (a). Representative images and results from three independent experiments are shown. NS, not significant, *** P < 0.001