Tumor suppressive function of Matrin 3 in the basal-like breast cancer

摘要

Comparison of MATR3 protein levels in a panel of basal-like/triple-negative human breast cancer cell lines. a Endogenous MATR3 protein levels were examined in a small panel of basal-like/triple-negative breast cancer cell lines by western blotting. The lower panel demonstrates the relative normalized MATR3 protein levels. b MATR3 was transiently overexpressed in MDA-MB-231 cells and the expression was determined by western blotting. The lower panel demonstrates the relative normalized MATR3 protein levels. c MATR3 was silenced by shRNAs and the expression was determined by western blotting. The lower panel demonstrates the relative normalized MATR3 protein levels. dIn vitro cell proliferation was compared between control and MATR3-overexpressing MDA-MB-231 and between control and MATR3-depleted BT549 cells. n = 4 per group. e Anchorage-independent growth was examined to compare in vitro tumorigenesis by soft agar colony formation assay. n = 4 per group. Statistical significance in d and e was determined by an unpaired t-test. Error bars are s.e.m