HOXD-AS1 facilitates cell migration and invasion as an oncogenic lncRNA by competitively binding to miR-877-3p and upregulating FGF2 in human cervical cancer

摘要

HOXD-AS1 was upregulated and correlated with worse prognosis in CC. a qRT-PCR analysis demonstrated that lncRNA HOXD-AS1 was significantly upregulated in cancerous tissues compared with in matched normal tissues. Expression levels of HOXD-AS1 were normalized to that of GAPDH. Data were calculated from triplicates. Bar value is the difference of HOXD-AS1 expression levels between tumors (T) and matched normal tissues (N) from the same patient. Bar value ≤ − 1 represents HOXD-AS1 is decreased in tumors. Bar value ≥1 represents that HOXD-AS1 is increased in tumors. b qRT-PCR analysis demonstrated that lncRNA HOXD-AS1 expression was higher in high stage (III + IV) CC tissues compared with in low stage (I + II) CC tissues. c Kaplan-Meier method with log-rank test indicated that patients with higher HOXD-AS1 expression had worse overall survival than those with lower HOXD-AS1 expression. d qRT-PCR analysis demonstrated that lncRNA HOXD-AS1 was significantly upregulated in CC cells (SiHa, CaSki, Hela, C-33A) compared with normal cervical epithelial cells (Ect1/E6E7). **: P < 0.01, ***: P < 0.001