Evolutionary engineering of Lactobacillus bulgaricus reduces enzyme usage and enhances conversion of lignocellulosics to D-lactic acid by simultaneous saccharification and fermentation

摘要

Effect of different enzyme loading on enzymatic cellulose hydrolysis. Experiment was conducted at 50 °C and pH 4.5 with 10% (w/v) pre-treated LCB (dil. acid pre-treated at 175 °C) at different enzyme loadings to evaluate cellulose hydrolysis (%) white bar-5 FPU/gLCB, Horizontally striped bar-10 FPU/gLCB, black bar-15 FPU/gLCB, Diagonally striped bar-30 FPU/gLCB Values are mean ± SD (n = 3)