Strategy for early callus induction and identification of anti-snake venom triterpenoids from plant extracts and suspension culture of Euphorbia hirta L.

摘要

Different callus morphologies: a callus growth after first subculture (bar 1 cm); b callus growth after second subculture (bar 1.5 cm); c mature, green, and friable callus (bar 2.5 cm); d pinkish red callus supplemented with NAA and BAP hormone (bar 3.5 cm) observed at different stages upon frequent subculturing