An optimized growth medium for increased recombinant protein secretion titer via the type III secretion system

摘要

Medium composition affects secretion titer. a Relative bulk secretion titer of SptP-DH-2xFLAG-6xHis in LB-L, 2X YT, and TB. b Relative bulk secretion titer of SptP-DH-2xFLAG-6xHis in LB-L supplemented with the defined components in TB. Statistical significance was determined by Welch’s unequal variances t-test. Significance denoted by “***” represents p < 0.001, while “n.s.” represents p > 0.05. c Relative bulk secretion titer versus relative expression in the media from parts a, b. Expression and secretion titer were normalized to LB-L with no carbon source using densitometry measurements on western blots. All error bars represent standard error for four biological replicates