Correction to: Short hairpin RNA- mediated gene knockdown of FOXM1 inhibits the proliferation and metastasis of human colon cancer cells through reversal of epithelial-to-mesenchymal transformation

摘要

Effect of altered FOXM1 expression on colorectal cancer cell migration and invasion in vitro. a: Wound healing assays were carried out at 24 h after transfection in 24-well plates, when cell confluence rate reached above 90% and a linear wound across the monolayer was done. The wound gap was photographed every 24 h, the gap width was measured (μm) using Open Lab software. b: The wound rate was calculated and displayed graphically as described in the Materials and Methods. c-d: SW620 cells of three groups were digested and resuspended in serum-free culture medium and allowed to migrate toward the lower chamber with coated or uncoated matrigel for 24 h. Invading cells were stained with 0.1% crystal violet and counted manually. C-Left: transwell migration assay, Right: transwell invasion assay. d: The number of invading SW620 cells by cell migration (upper) and invasion (lower) assay was counted manually. Each experiment was repeated thrice independently. Scale bar = 200 μm in those figures