Comparison of bias and resolvability in single-cell and single-transcript methods

摘要

a In this study, each cell culture is divided (split) to perform multiple methods in parallel for measuring the same underlying system of cellular sense and response. Eight cultures were measured over a range of induction with IPTG. Methods include various combinations of sample preparation, signal detection, and choice of measurand. Resolvability is quantitatively assessed using Area Under the receiver operator characteristic Curve (AUC) calculated across a range of IPTG concentrations. Relative bias is assessed by modeling cellular response using measurements from each method and comparing the resulting parameters. b Evaluation of resolvability with AUC profiles for all 12 methods. In each panel, the plotted symbols show the AUC for pairs of adjacent IPTG concentrations as indicated on the x-axis.