Dynamically remodeled hepatic extracellular matrix predicts prognosis of early-stage cirrhosis

摘要

A Microscopic views of livers from mice intraperitoneally injected with CCl4 for 1 day, 1 week or 4 weeks or olive oil for 4 weeks. Liver sections were stained with hematoxylin-eosin (HE), Picrosirius Red or immunostained for type I collagen (Col I) or fibronectin (FN). Scale bars: 100 μm. B–E Decellularized and enriched hepatic matrices were subjected to mass-spec analyses. B Numbers of matrisomal proteins identified at each liver fibrogenesis stage. C Venn diagram depicting overlap of matrisomal proteins across four liver fibrogenesis stages. D Gene ontology (GO) of identified matrisomal proteins. E Numbers of identified proteins in matrisomal classes. F Numbers of identified proteins in matrisomal classes at each liver fibrogenesis stage.