Evaluation of Antibiotic Dissemination into the Environment and Untreated Animals by Analysis of Oxytetracycline in Poultry Droppings and Litter

摘要

Oxytetracycline (OTC) is an antibiotic used mainly in feed and drinking water. OTC is poorly absorbed in the gastrointestinal tract of poultry; making droppings a potential route of dissemination of this antibiotic. The aim of this study was to evaluate the dissemination of oxytetracycline excreted from treated birds to the environment and other untreated animals (sentinels), through the analysis of their droppings and litter by HPLC-MS/MS following the end of treatment. In treated bird droppings, the average concentration of OTC+4-epi-OTC ranged from 347.63 to 2244.66 µg kg−1. OTC+4-epi-OTC in litter reached concentrations of 22,741.68 µg kg−1. Traces of OTC+4-epi-OTC were detected in the droppings and litter from sentinels. Therefore, OTC+4-epi-OTC can persist in the litter of treated animals at high concentrations and can be transferred to untreated birds that share the same environment. This exposure has the potential to increase the likelihood of selection of resistant bacteria in the environment.