Phenotypic Response of Wolbachia pipientis in a Cell-Free Medium

摘要

, an obligate intracellular bacterium estimated to infect millions of arthropod species worldwide, is currently being utilized in novel control strategies to limit the transmission of Dengue and Zika viruses. A limitation for -based control approaches is the difficulty of transferring to novel hosts and the lack of tools for the genetic transformation of due to the inability to culture outside the insect host cell in an axenic media. Here, we applied extracellular to phenotypic microarrays to measure the metabolic response of in media formulations with different pH levels and supplementation with Casamino acids. Results suggested a pH of 6.5–6.8 and showed that the supplementation of 1 mg/mL casamino acids increased the survival and longevity of in an axenic medium. In addition, phenotypic microarrays are a useful tool to measure the phenotypic response of under different media conditions, as well as determine specific components that may be required for an axenic medium. This study is an initial step toward the development of a potential axenic culture system.