Recombinant vesicular stomatitis virus glycoprotein carrying a foot-and-mouthdisease virus epitope as a vaccine candidate

摘要

Foot-and-mouth disease (FMD) is one of the most highly contagious animal diseases. In aneffort to overcome the drawbacks of the currently used inactivated foot-and-mouth diseasevirus vaccine, a novel recombinant protein carrying foot-and-mouth disease virus VP1 GHloop epitope linked to vesicular stomatitis virus glycoprotein was expressed in abaculovirus system. Its antigenicity was confirmed with ELISA using monoclonal antibodyagainst foot-and-mouth disease virus. Twice immunizations one month apart in field pigsresulted in a significant antibody increase compared to the glutathione S-transferasecarrier containing the same epitope and the commercial vaccine. To my knowledge, this isthe first report that the recombinant protein vaccine was superior to the current vaccine.Although further studies are required to examine their immunogenicity in a large number ofanimals, this study sheds light on the development of a novel recombinant protein vaccinethat could be easily produced in a general laboratory as an alternative to the current FMDvaccine, which requires a biosafety level 3 containment facility for vaccineproduction.