High fat diet worsens Alzheimer’s disease-related behavioral abnormalities and neuropathology in APP/PS1 mice but not by synergistically decreasing cerebral blood flow

摘要

Experimental design/timeline, and animal weight gain and food consumption. ( ) Schematic illustrating animal groups, timeline, measurements, and interventions. APP/PS1 and WT mice were allocated to a high fat diet (Hfd) or normal chow (NC) at 4 months of age. Animals received behavioral testing multiple times, as indicated. A small cohort from all four groups received cranial windows and cortical imaging at 11 months of age. The remaining cohort received additional behavioral testing, including before and after administration of α-Ly6G at 19 months, and then received cranial windows and were imaged before and after α-Ly6G treatment. All experimental groups were kept on their diet continuously from four months of age until euthanasia. ( ) Average mouse weight for each group as a function of animal age, beginning at 4 months of age, distinguishing male and female mice in each group. ( ) Average mass of food intake per mouse per day for all groups, distinguishing female (left) and male (right) animals.