Validation of barley 2OGO gene as a functional orthologue of Arabidopsis DMR6 gene in Fusarium head blight susceptibility

摘要

Screening of mutants created by pRD207 CRISPR gene editing vector. ( ) RFLP assay by II on WT and T plants. ( ) T7E1 assay on WT and T plants. ( ) RFLP assay by II to screen for homozygous T mutants. The 420 bp band indicates mutation of the target site while the 144 bp and 276 bp bands are consistent with products expected from the WT locus. Due to the sample size, the images were grouped from different gels, but all samples in the same image were electrophoresed at the same voltage for the same length of time. The 1Kb+ molecular weight marker and the WT sample were from the same gel.