首页> 外国专利> Diagnosis Marker Composition for Rice Fusarium Head Blight caused by Fusarium graminearum species complex using disease development-specific genes and Kit comprising the same

Diagnosis Marker Composition for Rice Fusarium Head Blight caused by Fusarium graminearum species complex using disease development-specific genes and Kit comprising the same

机译:利用疾病发展特异性基因诊断由禾谷镰刀菌种复合体引起的水稻镰刀病的诊断标记组合物和包含该组合物的试剂盒

摘要

The present invention relates to a marker composition for diagnosing the onset of a red fungus disease and a kit comprising the same, wherein the fungus belonging to the fungi belonging to the fungi belonging to the fungus family, The present invention provides a marker composition and a kit containing an oligonucleotide of an optimal test gene capable of determining infection and pathogenesis of a pathogenic bacterium at the onset stage of a fusarium head blight. The present invention relates to a kit for identifying oligonucleotides complementary to normalized test genes used in real-time quantitative PCR under various conditions and effectively detecting the onset caused by the bacteria.The present invention relates to a method for detecting a fungus such as Fusarium germinia room, Fusarium asiaticum, Fusarium bushii fungus, Fusarium spp., Which are causative agents of rice spider blight, using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) In addition, we identified the specific genes for the pathogenesis stage to which qRT-PCR can be applied, and tested the possibility of infecting host plants and pathogenesis more rapidly by pathogenic red fungi using complementary primers Can be.;
机译:本发明涉及一种用于诊断红木耳病发病的标记物组合物和包含该组合物的试剂盒,其中属于真菌的真菌属于真菌家族的真菌,本发明提供了一种标记物组合物和其。试剂盒,其中包含最佳测试基因的寡核苷酸,该寡核苷酸能够确定镰刀菌枯萎病发作阶段的致病细菌的感染和发病机理。本发明涉及一种试剂盒,该试剂盒用于在各种条件下鉴定与实时定量PCR中使用的标准化测试基因互补的寡核苷酸,并有效地检测由细菌引起的发作。本发明涉及一种用于检测诸如镰刀菌(Fusarium)菌种的真菌的方法。室,使用定量实时逆转录聚合酶链反应(qRT-PCR),是稻瘟病的病原体,亚洲镰刀菌,灌木镰刀菌,镰刀菌属(Fusarium spp。)此外,我们还确定了致病阶段的特定基因可以应用哪种qRT-PCR,并使用互补引物测试致病性红色真菌更快速地感染宿主植物和发病机理的可能性。

著录项

  • 公开/公告号KR101855955B1

    专利类型

  • 公开/公告日2018-05-11

    原文格式PDF

  • 申请/专利权人 순천향대학교 산학협력단;

    申请/专利号KR20160006376

  • 发明设计人 윤성환;김희경;

    申请日2016-01-19

  • 分类号C12Q1/68;

  • 国家 KR

  • 入库时间 2022-08-21 12:37:51

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