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Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human

机译:骆驼和人类中东呼吸综合征冠状病毒(MERSCoV)的分子研究和系统发育分析

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摘要

Middle East Respiratory Syndrome Corona Virus (MERSCoV) have been reported in Arabian peninsula and sporadic cases in Europe and Asia. This study was conducted to evaluate the genetic analysis of this virus in human and camel at the first time in Iraq. Two hundred samples were collected from camels and human who suffering from respiratory symptoms, these samples treated with RNA extraction kit then amplification the genetic material by PCR which give 5% positive results. The amplicon then sequenced, registration in gene bank of NCBI for getting accession numbers. The local strains give close relationship with neighbor countries as Saudi Arabia and Jordan strains when using MEGA analysis software.
机译:中东半岛上已出现中东呼吸综合症冠状病毒(MERSCoV),欧洲和亚洲也有零星病例。这项研究的目的是在伊拉克首次评估这种病毒在人和骆驼中的遗传分析。从骆驼和患有呼吸道症状的人类中采集了200个样品,这些样品用RNA提取试剂盒处理,然后通过PCR扩增了遗传物质,得到5%的阳性结果。然后将扩增子测序,在NCBI的基因库中注册以获得登录号。使用MEGA分析软件时,本地菌株与沙特阿拉伯和约旦的菌株与邻国紧密联系。

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