Correlation between terminal restriction fragments and flow‐FISH measures in samples over wide range telomere lengths

摘要

Terminal restriction fragment ( ) analysis of human telomeres was used to calibrate flow‐fluorescence hybridization ( ) measures of telomere lengths to expand the range of measures and increase power of resolution of our previously published protocol. data used as the gold standard should be obtained by electrophoresis with suitable resolution applied to appropriately isolated genomic . When we considered attained by correct methods, we found our method to be insufficiently accurate, thus we have reviewed our previously published protocol to obtain the best coefficient of determination ( ) between our experimental results and valid lengths.