Single Lysis‐Salting Out Method of Genomic DNA Extraction From Dried Blood Spots

机译：从干血斑中提取基因组DNA的单一裂解盐析方法

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摘要

Dried blood spots (DBS) are an important form of bio‐sampling and valuable approach for storing blood samples for genetic studies. This has necessitated in developing an effective protocol to isolate genomic DNA (gDNA) from DBS samples.In this study, we have elucidated a dependable and non‐hazardous “single lysis‐salting out” (SLSO) protocol of gDNA extraction from DBS and compared against the available commercial kits.

机译：干血斑（DBS）是生物采样的一种重要形式，也是用于遗传研究的血液样本存储的重要方法。这就需要开发一种有效的方案从DBS样品中分离基因组DNA（gDNA）。在本研究中，我们阐明了从DBS提取gDNA的可靠且无害的“单裂解-盐析”（SLSO）方案，并进行了比较。对照可用的商业工具包。

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期刊名称 Journal of Clinical Laboratory Analysis
作者
Muntaj Shaik; Devaraju Kuramkote Shivanna; Mahesh Kamate; Vedamurthy AB; Kruthika‐Vinod TP;
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作者单位

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年(卷),期 2016(30),6
年度 2016
页码 -1
总页数 4
原文格式 PDF
正文语种
中图分类生理学;
关键词
guthrie cards; DNA isolation; genotyping; GCDH; R402W mutation;

机译：guthrie卡;DNA分离;基因分型;GCDH;R402W突变;

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专利

1. Single Lysis-Salting Out Method of Genomic DNA Extraction From Dried Blood Spots [J] . Shaik Muntaj, Shivanna Devaraju Kuramkote, Kamate Mahesh, Journal of clinical laboratory analysis. . 2016,第6期

机译：从干血斑中提取基因组DNA的单裂解-盐析方法
2. Single Lysis‐Salting Out Method of Genomic DNA Extraction From Dried Blood Spots [J] . Muntaj Shaik, Devaraju Kuramkote Shivanna, Mahesh Kamate, Journal of clinical laboratory analysis. . 2016,第6期

机译：从干血斑中提取基因组DNA的单一裂解盐析方法
3. A simple method for extraction and purification of genomic DNA from dried blood spots on filter paper. [J] . Chaisomchit S, Wichajarn R, Chowpreecha S, The Southeast Asian journal of tropical medicine and public health . 2003,第3期

机译：一种从滤纸上干血斑中提取和纯化基因组DNA的简单方法。
4. An Efficiency Human Genomic DNA Extraction from Dried Blood Spots [C] . Nguyen Thi Hue, Phan Tuan Phong, Nguyen Dieu Hoai Chan International Conference on Environment Science and Biotechnology . 2013

机译：干燥血斑效率的人类基因组DNA提取
5. Development of Newborn Screening Methods for Mucopolysaccharidosis III Type A and Type B in Dried Blood Spot Using Tandem Mass Spectrometry [D] . Yi, Fan. 2018

机译：使用串联质谱法，在干血液点粘性型A和型B型和型B的新生筛查方法的开发
6. Combination of PURE-DNA extraction and LAMP-DNA amplification methods for accurate malaria diagnosis on dried blood spots [O] . Jeanne Perpétue Vincent, Kanako Komaki-Yasuda, Moritoshi Iwagami, 2018

机译：结合PURE-DNA提取和LAMP-DNA扩增方法对干血斑进行准确的疟疾诊断
7. Improvement of DNA Extraction Method for Dried Blood Spots and Comparison of Four PCR Methods for Detection of Babesia gibsoni (Asian Genotype) Infection in Canine Blood Samples [O] . Hiroyuki TANI, Yuji TADA, Kazumi SASAI, 2008

机译：干血斑DNA提取法的改进及四种PCR方法检测犬血液样本中BABESIA GIBSONI（亚洲基因型）感染的比较

Single Lysis‐Salting Out Method of Genomic DNA Extraction From Dried Blood Spots

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