Factor V Leiden Prothrombin 20210GA MTHFR 677CT and 1298AC and Homocysteinemia in Tunisian Blood Donors

摘要

Specific genetic conditions are known to be associated with high risk of venous thromboembolism. This genetic basis varies widely between ethnic groups. We investigated the distribution of four inherited polymorphisms in 113 unselected unisian blood donors by using polymerase chain reaction‐restriction fragment length polymorphism ( ‐ ) method. The allele frequencies of Factor V eiden ( ), prothrombin 20210G> , methylenetetrahydrofolate reductase ( ) 677 > , and 1298 > mutations were 3, 0.9, 30, and 31%, respectively. The 677 > polymorphism was influenced by age. Twenty‐nine of the 113 blood donors demonstrated more than one genetic markers. Hyperhomocysteinemia was found in 12 subjects, and it was statistically associated to the 677 genotype. Principal component analysis allowed disclosing the resemblance between editerranean populations. Our findings may be helpful for population genetics study, and provide epidemiologic database for further studies in thrombosis field among unisians. J. Clin. Lab. Anal. 26:167‐173, 2012. © 2012 Wiley Periodicals, Inc.