Oncolytic Newcastle disease virus delivered by Mesenchymal stem cells-engineered system enhances the therapeutic effects altering tumor microenvironment

摘要

Differentiation and characterization of bone marrow mesenchymal stem cells (BM-MSCs). MSCs were isolated from the bone marrow of 6-8 week-old C57BL/6 mice by selective adherence to the plastic cell culture dishes. Adipogenic (see black arrow) and osteogenic (see white arrow) differentiation were evaluated by Oil Red O staining and Alizarin Red as described in the materials and methods. Flow cytometry quantification of MSCs after isolation and enrichment showed the MSCs were positive for CD44 and CD105 but negative for CD34 and CD45 markers. The cytotoxic effects of the different NDV MOIs on MSCs was evaluated by MTT assay at 72 h post-infection. Means are from 3 independent experiments and *(