Inhibition of LSD1 promotes the differentiation of human induced pluripotent stem cells into insulin-producing cells

摘要

Morphology and phenotype characteristics of pancreatic β cells derived from hiPSCs at the final maturation stage. Morphological changes of hiPSCs during differentiation into mature pancreatic β cells. The pancreatic β cells derived from hiPSCs were stained with DTZ. Scale bars, 500 μm (b1, b2, b3, b4). Scanning electron microscopy of IPCs derived from hiPSCs. The IPCs have secretory granules and complete capsules. Scale bars, 1 μm (c1); 0.5 μm (c2, c3). Immunofluorescence staining showing that the differentiated hiPSCs at the final mature stage co-expressed PDX1 and NKX6.1, insulin and glucagon, and PDX1 and insulin (scale bars = 50 μm)