CD90 serves as differential modulator of subcutaneous and visceral adipose-derived stem cells by regulating AKT activation that influences adipose tissue and metabolic homeostasis

摘要

S-ADSCs show higher potential in proliferation than V-ADSCs through promoting AKT activation. S-ADSCs and V-ADSCs from inguinal and epididymal adipose tissue of the mice (  = 10) were used in the following experiments. , Cell proliferation was determined by EdU incorporation assay. Fluorescence signals were examined by fluorescence microscope, and growth index (number of EdU-positive nucleiumber of all nuclei) was calculated. Representative ( ) and statistic ( ) data are shown. Scale bar 100 μm. , Colony formation was measured by crystal violet staining. Representative ( ) and statistic ( ) data are shown. Scale bar 8.5 mm. , Cell cycle profiles were analyzed by flow cytometry after PI staining. Representative ( ) and statistic ( ) data are shown. The expression of stemness genes was measured by qPCR. , Protein levels of p-AKT, AKT, and CyclinD1 were detected on S-ADSCs and V-ADSCs in the absence ( ) or presence ( ) of MK2206 (2 μM) for 20 h by western blot. Data are presented as mean ± SEM.  = 3–5 per group. *  P P