METHOD FOR ISOLATING AND EXTRACTING ADIPOSE-DERIVED STEM CELLS FROM ADIPOSE TISSUE AND CULTURING SAME WITHOUT USING COLLAGENASE, AND KIT FOR ISOLATING AND EXTRACTING ADIPOSE-DERIVED STEM CELLS

摘要

Adipose-derived stem cells are isolated and extracted from an adipose tissue without using collagenase.An adipose tissue is covered with a nonwoven fabric sheet having sufficient interfiber space and pressed by an appropriate force. Thus, the adipose tissue infiltrates among fibers of the nonwoven fabric and then comes into contact with fibers surrounding the same. By immersing the nonwoven fabric as such in a culture medium, a number of adipose-derived stem cells are allowed to crawl out from the adipose tissue and then adhered to the fiber surface.