A magic kick for regeneration: role of mesenchymal stromal cell secretome in spermatogonial stem cell niche recovery

摘要

Mesenchymal stromal cell (MSC) secretome injections stimulate recovery of the spermatogonial stem cell (SSC) niche by affecting the whole testicle. H&E-stained testicular tissue sections of the untreated group ( ), the MSC secretome group ( ), the unaltered control group ( ), and the MSC group ( ); scale bars = 100 μm. Percentages of atrophic seminiferous tubules ( ); data are presented as means ± standard deviations (SDs) of atrophic seminiferous tubules in three sections from every rat testicle. Quantitative description of the spermatogenic epithelial cell subpopulation. Primary spermatocytes. Secondary spermatocytes. Spermatozoa. Data are presented as mean cell numbers ± standard deviations (SDs) per 40x field of view in three independent sections per testicle. Dark gray bars, 1 month follow-up; bright gray, 3 months. For – , three animals were analyzed for each mean in untreated and MSC groups, and four animals in vehicle and MSC secretome groups. Percentages of female fertilized rats, 16 animals were analyzed in the untreated group, 17 in the MSC secretome group, and 14 in the group of unaltered control animals (dotted line)