Evaluation of secondhand smoke effects on CFTR function in vivo

摘要

Effect of secondhand smoke on CFTR function in vitro. Primary human bronchial epithelial (HBE) cells were isolated from healthy non-smokers expressing wild type CFTR (CFTR +/+) and cultured at air liquid interface (ALI) until terminally differentiated into ciliated epithelium. HBE cell monolayers were exposed to secondhand smoke (SHS) for 10 min and CFTR function was measured in Ussing chambers under voltage clamp conditions. Representative tracings of forskolin stimulated CFTR ion transport in HBE cells. Addition of forskolin (20 μM) and CFTR 172 (10 μM) are indicated. Summary of changes in CFTR-dependent anion transport are plotted for forskolin stimulation ( ) and inactivation by specific inhibitor, CFTR 172 ( ). Bar graph depicting transepithelial resistance in HBE cell monolayers after exposure to 10 min of air or SHS.  = 6, *  P