A small-scale fractionation pipeline for rapid analysis of seed mucilage characteristics

摘要

Schematic representation of pipeline used to extract small quantities of seed mucilage in discrete fractions for yield and chromatographic analysis. Seeds and water are added to a microcentrifuge tube and incubated in a thermomixer-type shaking incubator at 25 °C for 1.5 h with agitation. After centrifugation, the cold water extractable (CWE) mucilage is removed and water is added to the pellet to restore the working volume. The sample is incubated at 65 °C for 1.5 h with agitation and after centrifugation the hot water extractable (HWE) mucilage is removed. Water is added to the pellet to restore the working volume. Intense agitation by a mixer mill-type tissue disrupter is used to disrupt the seed-mucilage attachment. After centrifugation, the intense extraction extractable (IAE) mucilage is removed. The pellet contains the demucilaged seeds (DMS). All fractions can be freeze-dried for yield and chromatographic analysis