DDIAS promotes STAT3 activation by preventing STAT3 recruitment to PTPRM in lung cancer cells

摘要

Expression of DDIAS and phosphorylated STAT3 in several NSCLC cell lines. , Loss of DDIAS selectively suppressed the STAT3 Y705 phosphorylation in NCI-H1703 and NCI-H23 cells after IL-6 (20 ng/ml) and EGF (100 ng/ml) treatment for the indicated times. Immunocytochemistry for pSTAT3 (Y705) in DDIAS-depleted cells treated with IL-6. Scale bars represent 10 μm. Transactivation of STAT3 by IL-6. NCI-H1703 cells were transfected with siDDIAS and M67-Luc, treated with IL-6 for 6 h, and the relative luciferase activity was determined. The results shown are representative of three experiments (  = 3). The values represent means ± SEM. **  f Overexpression of DDIAS enhanced the STAT3 Y705 phosphorylation in NCI-H358 cells after IL-6 (20 ng/ml) treatment for the indicated times.