CUL4B contributes to cancer stemness by repressing tumor suppressor miR34a in colorectal cancer

摘要

Representative pictures of IHC straining of CUL4B in human CRC tissues and the adjacent normal tissues (left). The percentages of CUL4B-positive cells in 75 paired human colon tumor and their adjacent tissues (right). Data represent mean ± SEM (  = 75). ***  b Representative pictures of IHC straining of CUL4B in human CRC LNM absent tissues and LNM present tissues. Kaplan–Meier analysis of the correlations between CUL4B protein level and overall survival of 100 patients with CRC. CUL4B , the expression of CUL4B is over than 50% in group and CUL4B is the other 50%. **  d Representative pictures of five PDOs. Representative photographs were taken at ×40 magnification of organoids after the knockdown of in #02T PDOs and #09T PDOs or after the overexpression of CUL4B in #16T PDOs. Organoids formation assay showed organoid number per 15,000 cells in knockdown and control #09T PDOs or #02T PDOs and in CUL4B overexpression and control #16T PDOs cultured for 7–10 days. Data represent mean ± SEM (  = 4). **  p