FadA-positive Fusobacterium nucleatum is prevalent in biopsy specimens of Iranian patients with colorectal cancer

摘要

has been increasingly implicated as a causative agent of various diseases, such as inflammatory bowel disease. Moreover, the gastrointestinal tracts of patients with colorectal cancer (CRC) also have been shown to be colonized by this bacterium. We aimed to determine the prevalence of among CRC and non-CRC Iranian patients and to investigate potential associations between -positive and diagnosed CRC cases. Eighty patients admitted to two main hospitals in Tehran, Iran, were enrolled. The patients were aged between 20 and 75 and were diagnosed by a gastroenterologist. A trained surgeon used standard surgical protocols to collect two CRC biopsy samples per patient. One of the samples was used for pathologic examination, and the other was subjected to DNA extraction and PCR. Lesion colonization by and expression of its major virulence factor, were investigated. The -positive strain was absent in all the lesions obtained from non-CRC patients. All patients with lesions that were colonized with -positive were diagnosed as CRC (p F. nucleatum colonization and lesions from CRC patients (p  0.0001; odds ratio, 6.74; 95% confidence interval, 2.5–18.07). Our study confirmed colonization of the -positive on lesions from 80 Iranian CRC patients. New therapeutic strategies to achieve eradication of are necessary for clinical management of patients suspected of having or prone to developing CRC.