Inhibition of lung cancer growth and metastasis by DHA and its metabolite RvD1 through miR-138-5p/FOXC1 pathway

摘要

Exogeneous and endogenous DHA inhibit cell growth, invasion and metastasis of NSCLC cells. . A549, H1299 or LLC cells were treated with various concentration of DHA, and were subjected to cell growth and invasion assays (  = 4). *, ,  ##,  b. LLC cells were transduced with lentivirus carrying the mfat-1 gene, or GFP as a negative control. Components were identified by comparison of retention times with those of PUFA analytical standard. The amount of DHA is expressed as a percentage of all fatty acid peaks. . LLC-GFP and LLC-mfat-1 were subjected to cell growth and invasion assays (  = 4). . LLC cells were subcutaneously injected into female WT or mfat-1 mice (  = 8). Survival of tumor-bearing mice (Right). . Representative images of implantation tumors (Left). HE staining of tumors (400×) (Right). . LLC cells were injected into tail veins of female WT or mfat-1 mice (  = 8). Survival of tumor-bearing mice. . CT images of metastatic lung tumors. . Mass of excised tumors. . HE staining of tumors (100×)