Characterization of the spatially-resolved expression of key proteins within tissues enables a deep understanding of biological systems. There has been significant progress in developing technologies with expanded capabilities to analyze higher numbers of proteins, however, the validation of these technologies and their associated affinity reagents remains a significant barrier to adoption. We have developed a validation pipeline that ensures optimal sensitivity and specificity for high-plex antibody panels for the analysis of FFPE sections using the NanoString GeoMx Digital Spatial Profiling (DSP) platform. The DSP is designed to simultaneously analyze up to 96 proteins by detecting oligos conjugated to antibodies that can be released via a UV-cleavable linker.
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