Downregulation of miR-204 is associated with poor prognosis and promotes cell proliferation in hypopharyngeal squamous cell carcinoma (HSCC)

摘要

MircroRNAs (miRNAs) were identified to be involved in tumor progression and prognosis. However, the clinical significance and biological function of miR-204 in hypopharyngeal squamous cell carcinoma (HSCC) are not well appreciated. Therefore, the aim of this study is to explore the role of miR-204 in HSCC. The miR-204 expression was determined in 56 pairs of HSCC tumor and adjacent non-tumor tissues by quantitative real-time reverse transcriptive-PCR (qRT-PCR). Kaplan-Meier survival curve analysis and log-rank test were used to analyze the association between miR-204 expression and clinicopathological parameters and the over survival (OS) time in HSCC patients. Univariate and multivariate Cox analysis was applied to investigate the predicted risk factors for OS. Moreover, CCK8 cell proliferation assays, flow cytometry analysis and western-blot analysis were performed to examine the cell growth and cell cycle related protein expression in FaDu cells. In the study, our results reported that miR-204 was down-regulated in HSCC tissues. The patients with lower miR-204 expression had significantly poor OS time. Multivariable Cox analysis demonstrated that lower miR-204 expression was an independent risk factor in HSCC patients. Furthermore, CCK8 cells assays and cell cycle analysis showed that over-expression of miR-204 significantly inhibited cell proliferation, S phase cell number and inhibited the cell cycle related protein expression of CyclinD1, CDK4 and CDK6, but up-regulated the p21 expression in FaDu cells. Thus, our study demonstrated that miR-204 was downregulated in HSCC and upregulation of miR-204 suppressed cell proliferation, which highlighted that miR-204 could have potential therapeutic applications in HSCC.