The Effects of Cyanobacterial Bloom Extracts on the Biomass Chl-a MC and Other Oligopeptides Contents in a Natural Planktothrix agardhii Population

摘要

Blooms of the cyanobacterium are common in shallow, eutrophic freshwaters. may produce hepatotoxic microcystins (MCs) and many other bioactive secondary metabolites belonging mostly to non-ribosomal oligopeptides. The aim of this work was to study the effects of two extracts (Pa-A and Pa-B) of -predominated bloom samples with different oligopeptide profiles and high concentration of biogenic compounds on another natural population. We hypothesised that the biomass and content of oligopeptides in is shaped in a different manner by diverse mixtures of metabolites of different -dominated cyanobacterial assemblages. For this purpose, the biomass, chlorophyll a and oligopeptides content in the treated were measured. Seven-day microcosm experiments with four concentrations of the extracts Pa-A and Pa-B were carried out. Generally, aeruginosins (AERs), cyanopeptolins (CPs) and anabaenopeptins (APs) were the most numerous peptides; however, only 16% of them were common for both extracts. The addition of the extracts resulted in similar effects on : an increase in biomass, Chl-a and MC content in the exposed as well as changes in its oligopeptide profile were observed. MCs present in the extracts did not inhibit accumulation of biomass, and did not have any negative effect on MC and Chl-a content. No evidence for bioaccumulation of dissolved peptides in the exposed was found. As the two tested extracts differed considerably in oligopeptide composition, but contained similar high concentrations of nutrients, it seems that biogenic compounds, not oligopeptides themselves, positively influenced the mixed natural population.