BMP4 and perivascular cells promote hematopoietic differentiation of human pluripotent stem cells in a differentiation stage-specific manner

摘要

A schematic overview of the stepwise hematopoietic induction protocol for hESCs (CHA15) and hiPSCs (hEF12-2 and iPS-NT4-S1). Representative bright-field images of colonies at the early (day 2) and late (day 17) stages of hematopoietic differentiation with BMP4 (20 vs 100 ng/mL). Scale bar, 200 μm. , Effects of low- and high-dose BMP4 on the induction efficiency ( ) and the yield ( ) of hematopoietic lineage cells from hPSCs were analyzed by flow cytometry. The bars indicate the mean ± SD; *  p