Structural basis for virulence regulation in Vibrio cholerae by unsaturated fatty acid components of bile

摘要

Structure of ToxT –unsaturated fatty acid (UFA) complex. Asymmetric unit of the ToxT (PDB 6P7R) structure aligned with the structure of ToxT (3GBG). ToxT is colored from the N-terminus to the C-terminus in dark blue to red. ToxT is colored gray. Close-up of the UFA-binding pocket of ToxT showing the sidechain interactions with the carboxylate head. Electron density is shown as the 2Fo-Fc map contoured to 1.5 σ. Structural alignment of the regulatory domain of UFA-bound ToxT to the AraC regulatory domain dimer (PDB 2ARA). AraC is colored gray, the regulatory domain of UFA-bound ToxT is colored blue to green. Structural alignment of the DNA-binding domain of UFA-bound ToxT to MarA in complex with DNA (PDB 1BL0). MarA is colored gray, the DNA-binding domain of UFA-bound ToxT is colored yellow to red.