Process for producing a vibrio cholerae thy a- strain, vibrio cholerae thy a- strain, nucleotide sequences of a vibrio cholerae thy a gene and a 5 'and 3' flank region of a thy a gene structural gene vibrio cholerae protein and vaccine

摘要

"PROCESS TO PRODUCE A THY A ^ - ^ VIBRIO CHOLERAE, CEPA THY A ^ - ^ VIBRIO CHOLERAE, NUCLEOTIDE SEQUENCES OF A GENE THY A OF VIBRIO CHOLERAE AND A FLANGE REGION 5 'AND 3' OF A GENE STRUCTURAL GENE THY A OF VIBRIO CHOLERAE, PROTEIN, AND, VACCINE "A process to produce a thy A ^ - ^ strain of cholera vibrion that comprises the site-directed mutagenesis stage of the V. cholerae chromosome at the thy gene site, A, SEQ ID NO: 1 of Figure 1 is described. Particularly a 30 thy A strain of Vibrio Cholerae that lacks thy A functionality is disclosed. This strain can comprise one or more episomic elements that autonomously replicate DNA, such as plasmids that have a functional gene in thy A, optionally foreign, for example, E. coli that allows the strain to develop in the absence of thymine in the development medium and which optionally has a structural gene encoding a homologous or heterologous protein. In addition, proteins encoded by a structural thy A gene and the 5 'flanking region are described as SEQ ID NO: 4 in Figure 4 and SEQ ID NO: 5 in Figure 5, respectively. Additionally a vaccine comprising a 30 thy A strain of Vibrio cholerae of the invention or a thy A strain of Vibrio cholerae produced by the process of the invention is disclosed.