Intracellular XBP1-IL-24 axis dismantles cytotoxic unfolded protein response in the liver

摘要

, WT mice were intraperitoneally injected with a single dose of CCL (2 ml/kg). GRP78, IL-24 and CHOP protein levels in the liver tissues were evaluated representatively by western blot at indicated time points. Results are normalized to β-actin.    3 independent experiments. IL-24 and CHOP mRNA levels in the liver tissues were assessed by qRT-PCR at indicated time points. Results are normalized to β-actin.    3 biological replicates. AML12 cells were exposed to Tm (5 µg/ml) for the indicated time period. IL-24 and CHOP levels were evaluated by western blot ( ) and qRT-PCR ( ) at indicated time points.    2 independent experiments ( ) or three biological replicates ( ). AML12 cells were transfected with indicated siRNAs or negative control (NC) 24 h prior to Tm treatment. IL-24 protein levels at indicated time points were assessed by western blot.    3 independent experiments. promoter activity in AML12 cells expressing indicated siRNAs, as quantified using luciferase assay. luciferase activity was normalized to firefly activity and presented as relative luciferase activity.  = 3 biological replicates. ChIP analysis of the promoter in Vector and sXBP1 overexpressing cells (Tm 0 h and 6 h) using flag antibodies. Data are presented as means ± SEM. *  P P P   0.0001. values were determined by two-tailed test.