Rheb1 protects against cisplatin-induced tubular cell death and acute kidney injury via maintaining mitochondrial homeostasis

摘要

Representative immunohistochemical staining images showing the induction of Rheb1 and p-S6 in kidney tubule from AKI patients. Scale bar = 20 μm. , Quantitative analyses of Rheb1 ( ) and p-S6-staining ( ) positive tubular cells in renal biopsies. ***  P n = 6. – Western blot assay (D) and quantitative analyses ( , ) showing the induction of Rheb1 and p-S6 in kidneys from mice after cisplatin injection,  = 4 for each point. Male CD1 mice were intraperitoneally injected with cisplatin at 20 mg/kg and kidney tissues were harvested at day 1, 2, and 3 thereafter. , Representative immunohistochemical staining images showing the induction of Rheb1 and p-S6 in kidney tubule from mice at day 1 after cisplatin injection. Scale bar = 20 μm.