TREM-1 multimerization is essential for its activation on monocytes and neutrophils

摘要

– U937 and U937-TD cells were stimulated with TREM-1 agonists αTREM-1 and Fab (10 µg/ml) with or without GαM (10 µg/ml) when indicated. Kinetics of intracellular calcium release in U937 cells. Left panel: TREM-1 expression on U937 (gray) and U937-TD (black) cells. Right panel: kinetics of intracellular calcium release in U937-TD cells. , Kinetics of intracellular calcium release in U937-TD cells incubated with increasing concentrations of αTREM-1 (0.1–20 µg/ml) and with αTREM-1 (5 µg/ml) and increasing concentrations of GαM (2.5–10 µg/ml). Western blot analysis of lysates of U937 and U937-TD cells at indicated times. IL-8 concentrations in supernatants after a 24-h stimulation. Data information: data are representative of at least three independent experiments. MFI mean fluorescence intensity, ns nonsignificant. *  p p t-test