IgH locus suicide recombination does not depend on NHEJ in contrast to CSR in B cells

摘要

DNA double-trand break repair in locus during locus suicide recombination. Repair structure is different between CSRγ1 and LSR junctions. Repair structure of total junctions (with insertions and microhomologies) is shown for CSRγ1 and LSR junctions. Repair structure of CSRγ1 and LSR junctions with insertions. Repair structure of CSRγ1 and LSR junctions with microhomologies. test: *  P P d XRCC4 is associated with Sµ and PARP-1 is recruited to LSR concerned regions (Sµ, hs1,2, and hs4). Fold enrichment (FE) of DNA repair proteins at regions concerned by LSR recombination (Sµ, hs1,2, and hs4) and CSRγ1 recombination (Sµ, Sγ1). Sγ3 was used as a negative control. Fold enrichment is shown for XRCC4 (  = 5 or 6), RAD51 (  = 5), PARP-1 (  = 3 or 4), and Polθ (  = 3). Histograms show means ± SEM. Unpaired test: ns nonsignificant, *  P