Novel alleles at the Gli-Bl locus generated by homologous recombination in the absence of the Phi locus.

摘要

A terminal segment of chromosome IBS containing storage protein locus Gli-Bl was saturated with a large number of crossovers in the presence and absence of the Phi locus. In the phlb background, eleven new alleles of Gli-Bl were identified. These included nulls, hybrid alleles composed of some proteins contributed by each parent, and duplicated alleles. The pattems of exchanges suggested that either parental loci were in non-corresponding positions or that out-of register crossovers were permitted, and that genes encoding proteins of similar gel mobility in each allele were ordered differently. Given the high stringency requirements imposed on crossing over in wheat by the Phi locus, such differences between the alleles might have prevented intralocus recombination in its presence.